luciferase reporter plasmid Search Results


nfat luciferase reporter  (Addgene inc)
93
Addgene inc nfat luciferase reporter
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
Nfat Luciferase Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/NFAT+luciferase+reporter+(Plasmid+%2310959)/pmc10410063-217-0-6
Average 93 stars, based on 1 article reviews
nfat luciferase reporter - by Bioz Stars, 2026-10
93/100 stars
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gal4uas luciferase reporter  (Addgene inc)
93
Addgene inc gal4uas luciferase reporter
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
Gal4uas Luciferase Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/GAL4UAS-Luciferase+reporter+(Plasmid+%2364125)/pmc05708895-230-4-6
Average 93 stars, based on 1 article reviews
gal4uas luciferase reporter - by Bioz Stars, 2026-10
93/100 stars
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4x clear luciferase reporter  (Addgene inc)
94
Addgene inc 4x clear luciferase reporter
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
4x Clear Luciferase Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/4XCLEAR-luciferase+reporter+(Plasmid+%2366800)/pmc06528971-222-6-16
Average 94 stars, based on 1 article reviews
4x clear luciferase reporter - by Bioz Stars, 2026-10
94/100 stars
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sgrna1 tet inducible luciferase reporter  (Addgene inc)
93
Addgene inc sgrna1 tet inducible luciferase reporter
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
Sgrna1 Tet Inducible Luciferase Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/sgRNA1_Tet-inducible+Luciferase+reporter+(Plasmid+%2364161)/pm41680487-183-21-33
Average 93 stars, based on 1 article reviews
sgrna1 tet inducible luciferase reporter - by Bioz Stars, 2026-10
93/100 stars
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tfeb promoter 46  (Addgene inc)
92
Addgene inc tfeb promoter 46
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
Tfeb Promoter 46, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/TFEB+promoter-luciferase+reporter+(Plasmid+%2366801)/pmc05576936-338-12-20
Average 92 stars, based on 1 article reviews
tfeb promoter 46 - by Bioz Stars, 2026-10
92/100 stars
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tet on inducible promoter  (Addgene inc)
92
Addgene inc tet on inducible promoter
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
Tet On Inducible Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/Tet-inducible+Luciferase+reporter+(Plasmid+%2364127)/pm36639706-452-11-21
Average 92 stars, based on 1 article reviews
tet on inducible promoter - by Bioz Stars, 2026-10
92/100 stars
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hu6 promoters  (Addgene inc)
93
Addgene inc hu6 promoters
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
Hu6 Promoters, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/hU6+promoter+PspCas13b+gRNA+dual+luciferase+reporter+3%E2%80%99UTR+(Plasmid+%23119859)/pmc12275094-295-13-21
Average 93 stars, based on 1 article reviews
hu6 promoters - by Bioz Stars, 2026-10
93/100 stars
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abca1 expression  (Addgene inc)
85
Addgene inc abca1 expression
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
Abca1 Expression, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/ABCA1+promoter+luciferase+reporter+(Full-length)+(Plasmid+%2386442)/pmc03058999-267-10-21
Average 85 stars, based on 1 article reviews
abca1 expression - by Bioz Stars, 2026-10
85/100 stars
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mouse acp5-luciferase reporter plasmid  (Promega)
90
Promega mouse acp5-luciferase reporter plasmid
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
Mouse Acp5 Luciferase Reporter Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/mouse+acp5+luciferase+reporter+plasmid/10__1074_slash_jbc__m313973200-46-0-9
Average 90 stars, based on 1 article reviews
mouse acp5-luciferase reporter plasmid - by Bioz Stars, 2026-10
90/100 stars
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plasmid containing a 2ere-luciferase reporter construct  (Promega)
90
Promega plasmid containing a 2ere-luciferase reporter construct
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
Plasmid Containing A 2ere Luciferase Reporter Construct, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/plasmid+containing+a+2ere+luciferase+reporter+construct/pmc03001348-80-11-23
Average 90 stars, based on 1 article reviews
plasmid containing a 2ere-luciferase reporter construct - by Bioz Stars, 2026-10
90/100 stars
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firefly luciferase reporter plasmid est-prom-luc  (Promega)
90
Promega firefly luciferase reporter plasmid est-prom-luc
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
Firefly Luciferase Reporter Plasmid Est Prom Luc, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/firefly+luciferase+reporter+plasmid+est+prom+luc/us09006206-599-7-40
Average 90 stars, based on 1 article reviews
firefly luciferase reporter plasmid est-prom-luc - by Bioz Stars, 2026-10
90/100 stars
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pmt2-gata4  (Promega)
90
Promega pmt2-gata4
Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. <t>NFAT</t> transcriptional activity evaluated by <t>luciferase</t> reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.
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Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. NFAT transcriptional activity evaluated by luciferase reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.

Journal: Aging Cell

Article Title: Reduction of class I histone deacetylases ameliorates ER ‐mitochondria cross‐talk in Alzheimer's disease

doi: 10.1111/acel.13895

Figure Lengend Snippet: Tac effect on InsP3R1‐VDAC1 interaction and MERCS morphology in AβO‐treated cells. HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. Representative confocal images of in situ PLA signal (gray) indicating a physical interaction between InsP3R1 and VDAC1 (scale bar = 20 μm). The lower panels represent enlargements of the boxed areas in the upper panels. Quantification of number of PLA puncta in about 200–240 cells from 8 image stacks per condition (a). WT hippocampal neurons were pre‐treated for 1 h with Tac (0.5 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h or treated solely with Tac for 24 h. NFAT transcriptional activity evaluated by luciferase reporter assay in hippocampal neurons (b) and HT22 cells (c). Data are the mean ± SEM of 4 independent experiments, run in triplicates to quadruplicates. Schematic representation of MERCS structural parameters: MERCS length and distance, mitochondrial perimeter and Feret's diameter (d). HT22 cells were pre‐treated for 1 h with Tac (10 μM) and then co‐incubated with 1 μM AβO for the remaining 23 h. Representative TEM images of mitochondria (in purple) in close contact with ER (in blue) (scale bar = 200 nm) in which distances ≤25 nm were considered as contacts (e). MERCS length (f) and distance (g), number of MERCS per mitochondria (h), mitochondrial area (i) and perimeter (j), Feret's diameter (k) and mitochondrial aspect ratio (l). Representative TEM images of mitochondrial cristae (in green) (scale bar = 200 nm) (m). Number of cristae per mitochondria (n), and cristae area (o). About 70–90 individual mitochondria from 10 randomly selected cells obtained from approximately 30 images were assessed in 26–38 independent TEM images. Statistical analysis: Kruskal–Wallis test followed by uncorrected Dunn's multiple comparisons test, one‐way ANOVA followed by uncorrected Fisher's LSD multiple comparison test; * p < 0.05; ** p < 0.01; *** p < 0.0001; **** p < 0.0001 when compared to control.

Article Snippet: NFAT luciferase reporter was obtained from Addgene.

Techniques: Incubation, In Situ, Activity Assay, Luciferase, Reporter Assay, Comparison, Control